The increasing global demand for house crickets (Acheta domesticus) necessitates effective health measures.Despite their abundance, the effects of gregarines on these hosts remain underexplored.We present a method for Above Ground Product (Lighting) controlled gregarine infection and maintenance of gregarine-free cricket populations.
This system, adapted from prior work, is essential for experiments on gregarine infection dynamics, providing insights into parasite evolution and host-parasite interactions within the Apicomplexa group.This protocol includes rearing and maintenance of gregarine-free cricket populations for experimental purposes, gregarine 5 Piece King Panel Bedroom production for oocyst solution and cricket infection, and gregarine infection assessment.